In general, pipets are used to measure and transport very precise volumes of liquids between containers or solutions. The term micropipet is used to describe a pipette that is used to work with very small volumes, less than 1000 ul (1 ml). Research assistants, scientists, and medical laboratory workers are just some of the professionals that utilize pipets in their laboratories.
There are almost countless makes and models of micropipets. Some of the functional features that may differ between models include volume range, incremental units, mode of operation (electronic or mechanical), autoclavability, and various ergonomic design features. To choose a model that is best for your laboratory and the proposed activity it is important to consider these features.
Volume range - You need a micropipet capable of delivering the various volume measurements that are included in your activity. If more than one micropipet offers an appropriate range, choose the range such that the volume you are interested in measuring is nearer the low end of the range. Some micropipets do not have adjustable volume capabilities, these are referred to as fixed volume pipettes.
Incremental units - Adjustable micropipettes can be adjusted for every 1ul, 2ul, 5ul, ect. The smaller the incremental unit the more precise the micropipette’s measurements will be.
Mode of operation - Micropipets may be operated electronically, usually by battery or AC power, or manually. Manual operation is most common, but electronic operation may be especially important to reduce operator fatigue and operator error when dealing with a repetitive pipeting task such as aliquoting.
Autoclavability - Some, but not all, micropipets are able to be placed in an autoclave for sterilization. If your activity demands sterile technique and sterile instruments then an autoclavable pipette must be chosen.
Various ergonomic design features - Some of these features that make using a micropipet more comfortable and reduce the stress placed on the body while pipeting include easy tip loading and unloading capability, a comfort grip, right /left handed readability, size, and weight. These features are especially important to consider if the pipeting will be repetitive. More information on pipeting and general laboratory ergonomic considerations.
Pipeting Techniques and General Guidelines
There are many pipeting dos and don’ts that can greatly affect the accuracy and consistency of volume measurement. Pipeting does require fine motor skills making practice and determination a must.
Download Pipetting Techniques and General Guidelines (PDF, 61kb)
Sargent Welch Featured Micropipets
Additional information on the BioHit mLine.
These micropipets are sturdy yet lightweight and meet the strictest performance, ergonomic, and safety criteria. Unique loading mechanism allows tip to be loaded with constant force, ensuring the tip is correctly sealed and can be ejected with minimum force. Thermal insulation of internal components provides improved accuracy as well as the ability to be autoclaved. The fast and light volume adjustment and small increments allow optimal precision.
The mLine also features a clear display with large, easy-to-read numbers. A counter locking mechanism prevents accidental volume changes during pipeting. Color coded ID tags and color coded caps that come with each model allow for easy micropipet identification as well as tip selection.
Finally, the micropipets are easy to calibrate with the included calibration tool, which can also be used as a tube cap opener. They are also easy to clean and maintain without the use of a particular opening tool.
Additional Resources
